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In this study, we investigated the in vivo generation of CD4 + CAR T cells using CD4-targeted LV (CD4-LV). Administration of CD4-LV into NSG mice transplanted with human peripheral blood mononuclear cells (PBMCs) led to 40%-60% of human CD4 + lymphocytes being CAR positive while CD8 + cells remained CAR negative.
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As long as that cell survives or gives rise to new cells, the FeLV proviral DNA persists and leaves behind a blueprint for infectious virus. Studies using polymerase chain reaction (PCR) for detecting proviral DNA copies of FeLV indicate that most cats are unlikely to eliminate this infection.1,2.
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